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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATF4 Recombinant antibody, PBS Only (Capture)

ATF4 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230306B5

Cat no : 81798-2-PBS

Synonyms

TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATF4 Recombinant antibody (81798-2-RR)

    WB analysis using 81798-2-RR (same clone as 81798-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81798-2-RR (ATF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81798-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATF4 Recombinant antibody (81798-2-RR)

    IF Staining of HeLa using 81798-2-RR (same clone as 81798-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using ATF4 antibody (81798-2-RR, Clone: 230306B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81798-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using ATF4 Recombinant antibody (81798-2-RR)

    FC experiment of HEK-293 using 81798-2-RR (same clone as 81798-2-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug ATF4 Recombinant antibody (81798-2-RR, Clone:230306B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81798-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00113-1

    Cytometric bead array standard curve of MP00113-1

    Cytometric bead array standard curve of MP00113-1, ATF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81798-2-PBS. Detection antibody: 81798-5-PBS. Standard: Ag1279. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00113-3

    Cytometric bead array standard curve of MP00113-3

    Cytometric bead array standard curve of MP00113-3, ATF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81798-2-PBS. Detection antibody: 81798-4-PBS. Standard: Ag1279. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 81798-2-RR

    Affinity and Kinetic Characterization of 81798-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81798-2-RR against Human ATF4 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81798-2-PBS targets ATF4 as part of a matched antibody pair:

MP00113-1: 81798-2-PBS capture and 81798-5-PBS detection (validated in Cytometric bead array)

MP00113-3: 81798-2-PBS capture and 81798-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATF4 fusion protein Ag1279 相同性解析による交差性が予測される生物種
Full Name activating transcription factor 4 (tax-responsive enhancer element B67)
Calculated molecular weight 39 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC022088
Gene symbol ATF4
Gene ID (NCBI) 468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer.