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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG101 Polyclonal antibody

ATG101 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26562-1-AP

Synonyms

ATG101, Autophagy related protein 101, C12orf44

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ATG101 Polyclonal antibody (26562-1-AP)

    WB analysis of mouse brain using 26562-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26562-1-AP (ATG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATG101 Polyclonal antibody (26562-1-AP)

    IP experiment of HeLa using 26562-1-AP

    IP result of anti-ATG101 (IP:26562-1-AP, 4ug; Detection:26562-1-AP 1:300) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATG101 Polyclonal antibody (26562-1-AP)

    IHC staining of human liver cancer using 26562-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26562-1-AP (ATG101 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATG101 Polyclonal antibody (26562-1-AP)

    IHC staining of human liver cancer using 26562-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26562-1-AP (ATG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATG101 Polyclonal antibody (26562-1-AP)

    IHC staining of human liver cancer using 26562-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26562-1-AP (ATG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATG101 Polyclonal antibody (26562-1-AP)

    IHC staining of human liver cancer using 26562-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26562-1-AP (ATG101 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:800
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26562-1-AP is ATG101 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG101 fusion protein Ag22932 相同性解析による交差性が予測される生物種
Full Name chromosome 12 open reading frame 44
Calculated molecular weight 218 aa, 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC005151
Gene symbol ATG101
Gene ID (NCBI) 60673
RRIDAB_2880554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG101 antibody 26562-1-APDownload protocol
IHC protocol for ATG101 antibody 26562-1-APDownload protocol
IP protocol for ATG101 antibody 26562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biol Int

TFAM knockdown undermines SQSTM1 mRNA stability but retards autophagy flux and inhibits tumor cells proliferation under starvation conditions

Authors - Xu Jiang
View Article