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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG12 Polyclonal antibody

ATG12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30505-1-AP

Synonyms

FBR93, Autophagy-related protein 12, APG12-like, APG12L, APG12 like

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG12 Polyclonal antibody (30505-1-AP)

    WB analysis using 30505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATG12 Polyclonal antibody (30505-1-AP)

    WB analysis using 30505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, NIH/3T3 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30505-1-AP targets ATG12 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG12 fusion protein Ag32268 相同性解析による交差性が予測される生物種
Full Name ATG12 autophagy related 12 homolog (S. cerevisiae)
Calculated molecular weight 15 kDa
Observed molecular weight20 kDa, 50-60 kDa
GenBank accession numberBC012266
Gene symbol ATG12
Gene ID (NCBI) 9140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

Protocols

Product Specific Protocols
WB protocol for ATG12 antibody 30505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols