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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG12 Polyclonal antibody, PBS Only

ATG12 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30505-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG12 Polyclonal antibody (30505-1-AP)

    WB analysis using 30505-1-AP (same clone as 30505-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30505-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATG12 Polyclonal antibody (30505-1-AP)

    WB analysis using 30505-1-AP (same clone as 30505-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30505-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30505-1-PBS targets ATG12 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG12 fusion protein Ag32268 相同性解析による交差性が予測される生物種
Full Name ATG12 autophagy related 12 homolog (S. cerevisiae)
Calculated molecular weight 15 kDa
Observed molecular weight20 kDa, 50-60 kDa
GenBank accession numberBC012266
Gene symbol ATG12
Gene ID (NCBI) 9140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.