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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG13 Recombinant antibody (82718-3-RR)

    WB analysis using 82718-3-RR (same clone as 82718-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82718-3-RR (KIAA0652 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82718-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ATG13 Recombinant antibody (82718-3-RR)

    WB analysis of HEK-293 using 82718-3-RR (same clone as 82718-3-PBS)

    WB result of ATG13 antibody (82718-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG13 transfected HEK-293 cells. This data was developed using the same antibody clone with 82718-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using ATG13 Recombinant antibody (82718-3-RR)

    IHC staining of human ovarian cancer using 82718-3-RR (same clone as 82718-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82718-3-RR (ATG13 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82718-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82718-3-RR

    Affinity and Kinetic Characterization of 82718-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82718-3-RR against Human ATG13 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

ATG13 Recombinant antibody, PBS Only

ATG13 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82718-3-PBS
Clone No.3G14

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

KIAA0652, Autophagy-related protein 13, Autophagy related protein 13, 3G14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82718-3-PBS targets ATG13 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATG13 fusion protein Ag13090 相同性解析による交差性が予測される生物種
Full Name KIAA0652
Calculated molecular weight 57 kDa
Observed molecular weight70 kDa
GenBank accession numberBC001331
Gene Symbol ATG13
Gene ID (NCBI) 9776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75143
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)