• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

ATG14/Barkor (C-terminal) Polyclonal antibody, PBS Only

ATG14/Barkor (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24412-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using ATG14/Barkor (C-terminal) Polyclonal antibody (24412-1-AP)

    WB analysis of HeLa using 24412-1-AP (same clone as 24412-1-PBS)

    WB result of ATG14/Barkor (C-terminal) antibody (24412-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG14/Barkor (C-terminal) transfected HeLa cells. This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ATG14/Barkor (C-terminal) Polyclonal antibody (24412-1-AP)

    WB analysis of mouse brain using 24412-1-AP (same clone as 24412-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ATG14/Barkor (C-terminal) Polyclonal antibody (24412-1-AP)

    IHC staining of human brain using 24412-1-AP (same clone as 24412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ATG14/Barkor (C-terminal) Polyclonal antibody (24412-1-AP)

    IHC staining of human brain using 24412-1-AP (same clone as 24412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ATG14/Barkor (C-terminal) Polyclonal antibody (24412-1-AP)

    IHC staining of human liver using 24412-1-AP (same clone as 24412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ATG14/Barkor (C-terminal) Polyclonal antibody (24412-1-AP)

    IHC staining of human liver using 24412-1-AP (same clone as 24412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATG14/Barkor (C-terminal) Polyclonal antibody (24412-1-AP)

    IF Staining of HeLa using 24412-1-AP (same clone as 24412-1-PBS)

    Immunofluorescent analysis of HeLa cells using 24412-1-AP (ATG14/Barkor (C-terminal) antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24412-1-PBS targets ATG14/Barkor (C-terminal) in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG14/Barkor (C-terminal) fusion protein Ag19707 相同性解析による交差性が予測される生物種
Full Name KIAA0831
Calculated molecular weight 492 aa, 55 kDa
Observed molecular weight 65-68 kDa, 42 kDa
GenBank accession numberBC109090
Gene symbol ATG14
Gene ID (NCBI) 22863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Barkor, also named as ATG14, Atg14L and KIAA0831, is named as Beclin 1-associated autophagy-related key regulator protein. The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation, and Salmonella typhimurium amplification. A major band around 65-68 kDa and a light band around 42 kDa can be observed in western blot which may be caused by alternative splicing of ATG14. The specificity of ATG14 antibody has been tested by siRNA test.