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ATG16L1 Polyclonal antibody

ATG16L1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 19812-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, IBD10, WDR30

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse spleen tissue, MCF-7 cells, Jurkat cells, HEK-293T cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19812-1-AP targets ATG16L1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG16L1 fusion protein Ag13844 相同性解析による交差性が予測される生物種
Full Name ATG16 autophagy related 16-like 1 (S. cerevisiae)
Calculated molecular weight 607 aa, 68 kDa
Observed molecular weight 63-71 kDa
GenBank accession numberBC000061
Gene Symbol ATG16L1
Gene ID (NCBI) 55054
RRIDAB_10695631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ676U5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

Protocols

Product Specific Protocols
WB protocol for ATG16L1 antibody 19812-1-APDownload protocol
IP protocol for ATG16L1 antibody 19812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Autophagy

ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1

Authors - Weihong Xie
humanIF

Nat Commun

Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.

Authors - Shuang-Zhou Peng
humanWB

Autophagy

AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.

Authors - Ashwinkumar Subramenium Ganapathy
humanWB

Curr Biol

Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.

Authors - Anna Ulbricht
humanWB,IHC

Cancer Lett

Let-7i-5p promotes a malignant phenotype in nasopharyngeal carcinoma via inhibiting tumor-suppressive autophagy.

Authors - Bo You
humanWB

Clin Transl Immunology

Circular RNA TRAPPC6B inhibits intracellular Mycobacterium tuberculosis growth while inducing autophagy in macrophages by targeting microRNA-874-3p.

Authors - Hou-Long Luo
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