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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG16L1 Monoclonal antibody, PBS Only

ATG16L1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5G7

Cat no : 67943-1-PBS

Synonyms

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG16L1 Monoclonal antibody (67943-1-Ig)

    WB analysis using 67943-1-Ig (same clone as 67943-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lung tissue using ATG16L1 Monoclonal antibody (67943-1-Ig)

    WB analysis of rat lung using 67943-1-Ig (same clone as 67943-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ATG16L1 Monoclonal antibody (67943-1-Ig)

    WB analysis of HepG2 using 67943-1-Ig (same clone as 67943-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATG16L1 Monoclonal antibody (67943-1-Ig)

    IF Staining of HepG2 using 67943-1-Ig (same clone as 67943-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG16L1 antibody (67943-1-Ig, Clone: 1G5G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67943-1-PBS targets ATG16L1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATG16L1 fusion protein Ag14881 相同性解析による交差性が予測される生物種
Full Name ATG16 autophagy related 16-like 1 (S. cerevisiae)
Calculated molecular weight 607 aa, 68 kDa
Observed molecular weight68-75 kDa
GenBank accession numberBC000061
Gene symbol ATG16L1
Gene ID (NCBI) 55054
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)