ATG16L2 Polyclonal antibody
ATG16L2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 24322-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse spleen tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
The immunogen of 24322-1-AP is ATG16L2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATG16L2 fusion protein Ag19399 相同性解析による交差性が予測される生物種 |
| Full Name | ATG16 autophagy related 16-like 2 (S. cerevisiae) |
| Calculated molecular weight | 619 aa, 69 kDa |
| Observed molecular weight | 70 kDa, 30 kDa |
| GenBank accession number | BC137490 |
| Gene symbol | ATG16L2 |
| Gene ID (NCBI) | 89849 |
| RRID | AB_2879493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG16L2 antibody 24322-1-AP | Download protocol |
| IF protocol for ATG16L2 antibody 24322-1-AP | Download protocol |
| IP protocol for ATG16L2 antibody 24322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gene Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications. |
