Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 15 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
25155-1-AP targets ATG2B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18412 Product name: Recombinant human ATG2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1304-1555 aa of BC015016 Sequence: RDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVS 相同性解析による交差性が予測される生物種 |
| Full Name | ATG2 autophagy related 2 homolog B (S. cerevisiae) |
| Calculated molecular weight | 2078 aa, 233 kDa |
| Observed molecular weight | 240-250 kDa |
| GenBank accession number | BC015016 |
| Gene Symbol | ATG2B |
| Gene ID (NCBI) | 55102 |
| RRID | AB_2810874 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96BY7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATG2B antibody 25155-1-AP | Download protocol |
| IHC protocol for ATG2B antibody 25155-1-AP | Download protocol |
| WB protocol for ATG2B antibody 25155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol ATG9A protects the plasma membrane from programmed and incidental permeabilization.
| ||
Nat Commun The autophagy protein ATG9A enables lipid mobilization from lipid droplets.
| ||
Autophagy SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores. | ||
Autophagy Architecture of the ATG2B-WDR45 complex and an aromatic Y/HF motif crucial for complex formation. | ||
EMBO Rep A conserved ATG2-GABARAP family interaction is critical for phagophore formation. |
