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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG4A Polyclonal antibody

ATG4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27467-1-AP

Synonyms

APG4A, ATG4A, AUTL2, Autophagin 2, Cysteine protease ATG4A, hAPG4A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of L02 cells using ATG4A Polyclonal antibody (27467-1-AP)

    WB analysis of L02 using 27467-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 27467-1-AP (ATG4A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MKN-45 cells using ATG4A Polyclonal antibody (27467-1-AP)

    WB analysis of MKN-45 using 27467-1-AP

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 27467-1-AP (ATG4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ATG4A Polyclonal antibody (27467-1-AP)

    IHC staining of human stomach cancer using 27467-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27467-1-AP (ATG4A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ATG4A Polyclonal antibody (27467-1-AP)

    IHC staining of human stomach cancer using 27467-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27467-1-AP (ATG4A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inL02 cells, MKN-45 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27467-1-AP targets ATG4A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG4A fusion protein Ag26497 相同性解析による交差性が予測される生物種
Full Name ATG4 autophagy related 4 homolog A (S. cerevisiae)
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC061696
Gene symbol ATG4A
Gene ID (NCBI) 115201
RRIDAB_2880879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG4, a member of the cysteine protease family, plays an important role in autophagy induction. ATG4 enzymes cleave the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine that is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes. ATG4A, which may be a target for cancer prevention and intervention, has been reported to be related to breast tumorigenesis, lung cancer, ovarian cancer, cervical cancer and gastric cancer. (PMID: 27276686, 29435029, 28636635)

Protocols

Product Specific Protocols
WB protocol for ATG4A antibody 27467-1-APDownload protocol
IHC protocol for ATG4A antibody 27467-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis.

Authors - Zhenhong Ni
View Article