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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG4B Monoclonal antibody (67727-1-Ig)

    WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATG4B Monoclonal antibody (67727-1-Ig)

    WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using ATG4B Monoclonal antibody (67727-1-Ig)

    WB analysis of HuH-7 using 67727-1-Ig (same clone as 67727-1-PBS)

    WB result of ATG4B antibody (67727-1-Ig; 1:800; room temperature for 1.5 hours) with wild-type and ATG4B knockout HuH-7 cells. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ATG4B Monoclonal antibody (67727-1-Ig)

    WB analysis of HSC-T6 using 67727-1-Ig (same clone as 67727-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATG4B Monoclonal antibody (67727-1-Ig)

    IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATG4B Monoclonal antibody (67727-1-Ig)

    IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ATG4B Monoclonal antibody (67727-1-Ig)

    IHC staining of human colon using 67727-1-Ig (same clone as 67727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATG4B Monoclonal antibody (67727-1-Ig)

    IF Staining of HeLa using 67727-1-Ig (same clone as 67727-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATG4B antibody (67727-1-Ig, Clone: 1G5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ATG4B Monoclonal antibody, PBS Only

ATG4B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67727-1-PBS
Clone No.1G5A7

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Autophagy-related cysteine endopeptidase 1, Autophagin-1, AUT-like 1 cysteine endopeptidase, AUTL1, APG4B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67727-1-PBS targets ATG4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATG4B fusion protein Ag30510 相同性解析による交差性が予測される生物種
Full Name ATG4 autophagy related 4 homolog B (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC000719
Gene Symbol ATG4B
Gene ID (NCBI) 23192
RRIDAB_2882913
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4P1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.