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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG4C Polyclonal antibody

ATG4C Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20382-1-AP

Synonyms

APG4 C, APG4C, ATG4C, AUTL1, AUTL3, Autophagin 3, Cysteine protease ATG4C

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ATG4C Polyclonal antibody (20382-1-AP)

    WB analysis of Jurkat using 20382-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20382-1-AP (ATG4C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using ATG4C Polyclonal antibody (20382-1-AP)

    IHC staining of human heart using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ATG4C Polyclonal antibody (20382-1-AP)

    IHC staining of human heart using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ATG4C Polyclonal antibody (20382-1-AP)

    IHC staining of human testis using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ATG4C Polyclonal antibody (20382-1-AP)

    IHC staining of human testis using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATG4C Polyclonal antibody (20382-1-AP)

    IF Staining of HeLa using 20382-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATG4C antibody (20382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20382-1-AP targets ATG4C in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG4C fusion protein Ag14207 相同性解析による交差性が予測される生物種
Full Name ATG4 autophagy related 4 homolog C (S. cerevisiae)
Calculated molecular weight 458 aa, 52 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC033024
Gene symbol ATG4C
Gene ID (NCBI) 84938
RRIDAB_10666852
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG4C antibody 20382-1-APDownload protocol
IHC protocol for ATG4C antibody 20382-1-APDownload protocol
IF protocol for ATG4C antibody 20382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Autophagy

Analysis of ATG4C function in vivo

Authors - Isaac Tamargo-Gómez
View Article
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun
View Article