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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG4C Recombinant antibody (84146-1-RR)

    WB analysis using 84146-1-RR (same clone as 84146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84146-1-RR (ATG4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84146-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84146-1-RR

    Affinity and Kinetic Characterization of 84146-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84146-1-RR against Human ATG4C were performed. The affinity constant is 1.26 nM.

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ATG4C Recombinant antibody, PBS Only

ATG4C Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84146-1-PBS
Clone No.230359A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

AUTL3, AUTL1, ATG, APG4C, APG4 C

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84146-1-PBS targets ATG4C in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATG4C fusion protein Ag14207 相同性解析による交差性が予測される生物種
Full Name ATG4 autophagy related 4 homolog C (S. cerevisiae)
Calculated molecular weight 458 aa, 52 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC033024
Gene Symbol ATG4C
Gene ID (NCBI) 84938
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96DT6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Autophagy related 4C cysteine peptidase (ATG4C) is an autophagy regulator responsible for cleaving of pro-LC3 and delipidation of LC3 II.