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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG5 Monoclonal antibody (66744-1-Ig)

    WB analysis using 66744-1-Ig (same clone as 66744-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ATG5 Monoclonal antibody (66744-1-Ig)

    WB analysis of HepG2 using 66744-1-Ig (same clone as 66744-1-PBS)

    WB result of ATG5 antibody (66744-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HepG2 cells. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATG5 Monoclonal antibody (66744-1-Ig)

    WB analysis of HeLa using 66744-1-Ig (same clone as 66744-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ATG5 Monoclonal antibody (66744-1-Ig)

    IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ATG5 Monoclonal antibody (66744-1-Ig)

    IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ATG5 Monoclonal antibody (66744-1-Ig)

    IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ATG5 Monoclonal antibody (66744-1-Ig)

    IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ATG5 Monoclonal antibody, PBS Only

ATG5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66744-1-PBS
Clone No.2D3B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2D3B8, APG5, APG5 LIKE, APG5L, APG5-like

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66744-1-PBS targets ATG5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATG5 fusion protein Ag16685 相同性解析による交差性が予測される生物種
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Calculated molecular weight 32 kDa
Observed molecular weight 50-55 and 40-45 kDa
GenBank accession numberBC002699
Gene Symbol ATG5
Gene ID (NCBI) 9474
RRIDAB_2882092
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H1Y0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)