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  • Featured Product
  • KD/KO Validated

ATG5 Recombinant antibody

ATG5 Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5M5

Cat no : 81803-1-RR

Synonyms

Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE, APG5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG5 Recombinant antibody (81803-1-RR)

    WB analysis using 81803-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81803-1-RR (ATG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATG5 Recombinant antibody (81803-1-RR)

    WB analysis of HeLa using 81803-1-RR

    WB result of ATG5 antibody (81803-1-RR; 1:6000; room temperature for 1.5 hours) with wild-type and ATG5 knockout HeLa cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATG5 Recombinant antibody (81803-1-RR)

    IP experiment of HeLa using 81803-1-RR

    IP result of anti-ATG5 (IP:81803-1-RR, 4ug; Detection:81803-1-RR 1:2000) with HeLa cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATG5 Recombinant antibody (81803-1-RR)

    IHC staining of human ovary tumor using 81803-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATG5 Recombinant antibody (81803-1-RR)

    IHC staining of human ovary tumor using 81803-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81803-1-RR

    Affinity and Kinetic Characterization of 81803-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81803-1-RR against Human ATG5 were performed. The affinity constant is 42.4 nM.

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, A549 cells, NIH/3T3 cells, RAW 264.7 cells, HSC-T6 cells, PC-12 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81803-1-RR targets ATG5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATG5 fusion protein Ag0214 相同性解析による交差性が予測される生物種
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Calculated molecular weight 32 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC002699
Gene symbol ATG5
Gene ID (NCBI) 9474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band.

Protocols

Product Specific Protocols
WB protocol for ATG5 antibody 81803-1-RRDownload protocol
IHC protocol for ATG5 antibody 81803-1-RRDownload protocol
IP protocol for ATG5 antibody 81803-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols