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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG7 Polyclonal antibody, PBS Only

ATG7 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28413-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG7 Polyclonal antibody (28413-1-AP)

    WB analysis using 28413-1-AP (same clone as 28413-1-PBS)

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 28413-1-AP (ATG7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28413-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ATG7 Polyclonal antibody (28413-1-AP)

    IHC staining of human cervical cancer using 28413-1-AP (same clone as 28413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 28413-1-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28413-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28413-1-PBS targets ATG7 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG7 fusion protein Ag29172 相同性解析による交差性が予測される生物種
Full Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Calculated molecular weight 78 kDa
Observed molecular weight68-70 kDa
GenBank accession numberBC000091
Gene symbol ATG7
Gene ID (NCBI) 10533
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.