Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, U2OS cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
81760-1-RR targets ATG7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ATG7 fusion protein Ag0142 相同性解析による交差性が予測される生物種 |
| Full Name | ATG7 autophagy related 7 homolog (S. cerevisiae) |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 68-78 kDa |
| GenBank accession number | BC000091 |
| Gene Symbol | ATG7 |
| Gene ID (NCBI) | 10533 |
| RRID | AB_2935577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95352 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG7 antibody 81760-1-RR | Download protocol |
| IF protocol for ATG7 antibody 81760-1-RR | Download protocol |
| IP protocol for ATG7 antibody 81760-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|