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  • KD/KO Validated

ATG7 Recombinant antibody

ATG7 Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2M8

Cat no : 81760-1-RR

Synonyms

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ATG7 Recombinant antibody (81760-1-RR)

    WB analysis of HeLa using 81760-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 81760-1-RR (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ATG7 Recombinant antibody (81760-1-RR)

    WB analysis of HepG2 using 81760-1-RR

    WB result of ATG7 antibody (81760-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATG7 Recombinant antibody (81760-1-RR)

    IP experiment of HeLa using 81760-1-RR

    IP result of anti-ATG7 (IP:81760-1-RR, 4ug; Detection:81760-1-RR 1:2000) with HeLa cells lysate 1720 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATG7 Recombinant antibody (81760-1-RR)

    IF Staining of HepG2 using 81760-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG7 antibody (81760-1-RR, Clone: 2M8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 81760-1-RR

    Affinity and Kinetic Characterization of 81760-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81760-1-RR against Human ATG7 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, U2OS cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81760-1-RR targets ATG7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATG7 fusion protein Ag0142 相同性解析による交差性が予測される生物種
Full Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Calculated molecular weight 78 kDa
Observed molecular weight68-78 kDa
GenBank accession numberBC000091
Gene symbol ATG7
Gene ID (NCBI) 10533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Protocols

Product Specific Protocols
WB protocol for ATG7 antibody 81760-1-RRDownload protocol
IF protocol for ATG7 antibody 81760-1-RRDownload protocol
IP protocol for ATG7 antibody 81760-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols