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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG8L Recombinant antibody

ATG8L Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240533F5

Cat no : 83817-1-RR

Synonyms

GABARAPL1, GABA(A) receptor-associated protein-like 1, Early estrogen-regulated protein, ATG8, APG8L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG8L Recombinant antibody (83817-1-RR)

    WB analysis using 83817-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83817-1-RR (ATG8L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATG8L Recombinant antibody (83817-1-RR)

    IF Staining of HepG2 using 83817-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATG8L antibody (83817-1-RR, Clone: 240533F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inmouse liver tissue, mouse brain tissue, rat brain tissue, rat liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83817-1-RR is ATG8L Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATG8L fusion protein Ag1473 相同性解析による交差性が予測される生物種
Full Name GABA(A) receptor-associated protein like 1
Calculated molecular weight 14 kDa
Observed molecular weight16 kDa
GenBank accession numberBC009309
Gene symbol GABARAPL1
Gene ID (NCBI) 23710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine.

Protocols

Product Specific Protocols
WB protocol for ATG8L antibody 83817-1-RRDownload protocol
IF protocol for ATG8L antibody 83817-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols