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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG9A Monoclonal antibody (67096-1-Ig)

    WB analysis using 67096-1-Ig (same clone as 67096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67096-1-Ig (ATG9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using ATG9A Monoclonal antibody (67096-1-Ig)

    IHC staining of human thyroid cancer using 67096-1-Ig (same clone as 67096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67096-1-Ig (ATG9A antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using ATG9A Monoclonal antibody (67096-1-Ig)

    IHC staining of human thyroid cancer using 67096-1-Ig (same clone as 67096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67096-1-Ig (ATG9A antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATG9A Monoclonal antibody (67096-1-Ig)

    IF Staining of HepG2 using 67096-1-Ig (same clone as 67096-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67096-1-Ig (ATG9A antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

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ATG9A Monoclonal antibody, PBS Only

ATG9A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67096-1-PBS
Clone No.1D12E11

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67096-1-PBS targets ATG9A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATG9A fusion protein Ag24278 相同性解析による交差性が予測される生物種
Full Name ATG9 autophagy related 9 homolog A (S. cerevisiae)
Calculated molecular weight 837 aa, 94 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC065534
Gene Symbol ATG9A
Gene ID (NCBI) 79065
RRIDAB_2882401
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7Z3C6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)