Validation Data Gallery

Filter: AllWBIP

WB analysis of 3T3-L1 using 55190-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 55190-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 55190-1-AP
WB result of ATGL antibody (55190-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATGL transfected A431 cells.
WB analysis using 55190-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 55190-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 55190-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55190-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55190-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 55190-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 55190-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 55190-1-AP
IP result of anti-ATGL (IP:55190-1-AP, 4ug; Detection:55190-1-AP 1:500) with mouse testis tissue lysate 4000ug.

Featured Product
KD/KO Validated

ATGL Polyclonal antibody

ATGL Polyclonal Antibody for WB, IP, ELISA

Cat No. 55190-1-AP

Publications(66)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Adipose triglyceride lipase, PNPLA2, Calcium-independent phospholipase A2-zeta, Desnutrin, EC:3.1.1.3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (66)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse heart tissue, 3T3-L1 cells, A431 cells, HeLa cells, human heart tissue, mouse testis tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected in	mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 61 publications below
IHC	See 5 publications below
IF	See 8 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

55190-1-AP targets ATGL in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	patatin-like phospholipase domain containing 2
Calculated molecular weight	55 kDa
Observed molecular weight	45-55 kDa
GenBank accession number	NM_020376
Gene Symbol	ATGL
Gene ID (NCBI)	57104
RRID	AB_11182818
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96AD5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.

Protocols

Product Specific Protocols
WB protocol for ATGL antibody 55190-1-AP	Download protocol
IP protocol for ATGL antibody 55190-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Pineal Res Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin. Authors - Zhenhui Li View Article
human		Hepatology PMID: 29729186 Authors - Huixia Zhang View Article
mouse	WB	Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) Authors - Xinzhi Li View Article
mouse	WB	Food Chem Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity. Authors - Shanshan Hu View Article
mouse	WB	Biochim Biophys Acta Mol Basis Dis Lipodystrophic gene Agpat2 deficiency aggravates hyperlipidemia and atherosclerosis in Ldlr-/- mice Authors - Kenan Peng View Article
human,rat	WB	Front Pharmacol Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism Authors - Tian Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of 3T3-L1 using 55190-1-AP

WB analysis using 55190-1-AP

WB analysis of A431 using 55190-1-AP

WB analysis using 55190-1-AP

WB analysis of rat heart using 55190-1-AP

WB analysis of mouse testis using 55190-1-AP

WB analysis of HeLa using 55190-1-AP

WB analysis of HeLa using 55190-1-AP

WB analysis of A431 using 55190-1-AP

WB analysis of human heart using 55190-1-AP

IP experiment of mouse testis using 55190-1-AP

ATGL Polyclonal antibody

ATGL Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin.

PMID: 29729186

Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)

Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.

Lipodystrophic gene Agpat2 deficiency aggravates hyperlipidemia and atherosclerosis in Ldlr-/- mice

Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism