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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATIC Recombinant antibody (84798-5-RR)

    WB analysis using 84798-5-RR (same clone as 84798-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84798-5-RR (ATIC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ATIC Recombinant antibody (84798-5-RR)

    IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ATIC Recombinant antibody (84798-5-RR)

    IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ATIC Recombinant antibody (84798-5-RR)

    FC experiment of HeLa using 84798-5-RR (same clone as 84798-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATIC Recombinant antibody (84798-5-RR, Clone:242324C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

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ATIC Recombinant antibody, PBS Only

ATIC Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84798-5-PBS
Clone No.242324C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

AICARFT, AICAR transformylase/inosine monophosphate cyclohydrolase, AICAR formyltransferase, AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84798-5-PBS targets ATIC in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATIC fusion protein Ag1052 相同性解析による交差性が予測される生物種
Full Name 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Calculated molecular weight 65 kDa
Observed molecular weight62 kDa
GenBank accession numberBC008879
Gene Symbol ATIC
Gene ID (NCBI) 471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31939
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

5-Aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC, also known as AICAR) is an enzyme that can catalyze the last two reactions in the de novo purine biosynthetic pathway. It has been previously reported to be upregulated and to participate in myeloma and hepatocellular carcinoma progression (PMID: 35251351).