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KD/KO Validated

ATL2 Polyclonal antibody

Name: ATL2 Polyclonal antibody
Brand: Proteintech
SKU: 16688-1-AP

ATL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16688-1-AP

Synonyms

ARL-6-interacting protein 2, ARL3IP2, ARL 6 interacting protein 2, Aip-2, Aip 2

Validation Data Gallery

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AllWBIHC

WB analysis using 16688-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 16688-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16688-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16688-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human intrahepatic cholangiocarcinoma using 16688-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16688-1-AP (ATL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 16688-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16688-1-AP (ATL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 16688-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16688-1-AP (ATL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 16688-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16688-1-AP (ATL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 16688-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, A549 cells, HeLa cells, Jurkat cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected in	human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IF	See 2 publications below

Product Information

16688-1-AP targets ATL2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATL2 fusion protein Ag10030 相同性解析による交差性が予測される生物種
Full Name	atlastin GTPase 2
Calculated molecular weight	412aa,47 kDa; 583aa,66 kDa
Observed molecular weight	66 kDa
GenBank accession number	BC053508
Gene symbol	ATL2
Gene ID (NCBI)	64225
RRID	AB_1850898
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATL2, also known as Atlastin GTPase 2, is involved in various cellular processes, including the organization of the Golgi apparatus, the endoplasmic reticulum tubular network membrane organization, and protein homooligomerization. It is located in the endoplasmic reticulum tubular network membrane and is an integral component of the membrane. The ATL2 gene has multiple transcripts and isoforms, with 18 transcripts (splice variants), 297 orthologues, and 10 paralogues. ATL2 also plays a significant role in the immune response, particularly in plants. In Arabidopsis thaliana, the ATL2 gene is involved in the response to fungal pathogen infection. The expression of ATL2 is low under normal growth conditions but is rapidly and significantly induced by exogenous chitin.also plays

Protocols

Product Specific Protocols
WB protocol for ATL2 antibody 16688-1-AP	Download protocol
IHC protocol for ATL2 antibody 16688-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	J Cell Biol Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. Authors - Nan Liu View Article
human,mouse,monkey	WB	J Cell Biol Human atlastin-3 is a constitutive ER membrane fusion catalyst Authors - Samantha Bryce View Article
mouse	WB	Nature Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy Authors - Hector Foronda View Article
human	WB	Nat Struct Mol Biol Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis Authors - Wei Zhang View Article
human	WB,IF	J Biochem Lunapark ubiquitinates atlastin-2 for the tubular network formation of the endoplasmic reticulum. Authors - Toshiaki Sakisaka View Article
mouse	WB	NPJ Parkinsons Dis Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities Authors - Jia Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATL2 Polyclonal antibody

ATL2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 16688-1-AP

WB analysis using 16688-1-AP

WB analysis of Jurkat using 16688-1-AP

WB analysis of HeLa using 16688-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 16688-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 16688-1-AP

IHC staining of human ovary cancer using 16688-1-AP

IHC staining of human ovary cancer using 16688-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.

Human atlastin-3 is a constitutive ER membrane fusion catalyst

Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy

Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis

Lunapark ubiquitinates atlastin-2 for the tubular network formation of the endoplasmic reticulum.

Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities