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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATM Monoclonal antibody (67586-1-Ig)

    WB analysis using 67586-1-Ig (same clone as 67586-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67586-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ATM Monoclonal antibody (67586-1-Ig)

    WB analysis of HSC-T6 using 67586-1-Ig (same clone as 67586-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67586-1-PBS in a different storage buffer formulation.

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ATM Monoclonal antibody, PBS Only

ATM Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67586-1-PBS
Clone No.3E6F4

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated, 3E6F4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67586-1-PBS targets ATM in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATM fusion protein Ag30001 相同性解析による交差性が予測される生物種
Full Name ataxia telangiectasia mutated
Observed molecular weight 310-350 kDa
GenBank accession numberBC137169
Gene Symbol ATM
Gene ID (NCBI) 472
RRIDAB_2882795
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13315
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.