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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATM Monoclonal antibody, PBS Only

ATM Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E6F4

Cat no : 67586-1-PBS

Synonyms

A T mutated, AT1, ATA, ataxia telangiectasia mutated, ATC, ATD, ATDC, ATE, ATM, DKFZp781A0353, Serine protein kinase ATM, TEL1, TELO1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATM Monoclonal antibody (67586-1-Ig)

    WB analysis using 67586-1-Ig (same clone as 67586-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67586-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ATM Monoclonal antibody (67586-1-Ig)

    WB analysis of HSC-T6 using 67586-1-Ig (same clone as 67586-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67586-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATM Monoclonal antibody (67586-1-Ig)

    IF Staining of HeLa using 67586-1-Ig (same clone as 67586-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67586-1-Ig (ATM antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67586-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67586-1-PBS targets ATM in WB, IF, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATM fusion protein Ag30001 相同性解析による交差性が予測される生物種
Full Name ataxia telangiectasia mutated
Observed molecular weight 310-350 kDa
GenBank accession numberBC137169
Gene symbol ATM
Gene ID (NCBI) 472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.