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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATM Recombinant antibody, PBS Only (Detector)

ATM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240560H8

Cat no : 83608-4-PBS

Synonyms

Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated, 240560H8

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00592-4

    Sandwich ELISA standard curve of MP00592-4

    Sandwich ELISA standard curve of MP00592-4, Human ATM Recombinant Matched Antibody Pair - PBS only. 83608-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25920. 83608-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP00592-4

    Sample test of MP00592-4

    The mean ATM concentration was determined to be 3,383.80 pg/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 3,536.69 pg/mL in MDA-MB-468 cell extract based on a 3.20 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATM Recombinant antibody (83608-4-RR)

    IF Staining of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (83608-4-RR, Clone: 240560H8 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ATM Recombinant antibody (83608-4-RR)

    FC experiment of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATM Recombinant Antibody (83608-4-RR, Clone:240560H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using ATM Recombinant antibody (83608-4-RR)

    FC experiment of MCF-7 using 83608-4-RR (same clone as 83608-4-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATM Recombinant Antibody (83608-4-RR, Clone:240560H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00592-1

    Cytometric bead array standard curve of MP00592-1

    Cytometric bead array standard curve of MP00592-1, ATM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83608-1-PBS. Detection antibody: 83608-4-PBS. Standard: Ag25920. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00592-3

    Cytometric bead array standard curve of MP00592-3

    Cytometric bead array standard curve of MP00592-3, ATM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83608-2-PBS. Detection antibody: 83608-4-PBS. Standard: Ag25920. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83608-4-RR

    Affinity and Kinetic Characterization of 83608-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83608-4-PBS targets ATM as part of a matched antibody pair:

MP00592-1: 83608-1-PBS capture and 83608-4-PBS detection (validated in Cytometric bead array)

MP00592-3: 83608-2-PBS capture and 83608-4-PBS detection (validated in Cytometric bead array)

MP00592-4: 83608-6-PBS capture and 83608-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATM fusion protein Ag25920 相同性解析による交差性が予測される生物種
Full Name ataxia telangiectasia mutated
GenBank accession numberBC137169
Gene symbol ATM
Gene ID (NCBI) 472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.