Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, Raji cells, MCF-7 cells, A549 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells, MCF-7 cells |
Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83608-5-RR targets ATM in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | ATM fusion protein Ag25920 相同性解析による交差性が予測される生物種 |
Full Name | ataxia telangiectasia mutated |
Observed molecular weight | 310-350 kDa |
GenBank accession number | BC137169 |
Gene Symbol | ATM |
Gene ID (NCBI) | 472 |
RRID | AB_3671222 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q13315 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.
Protocols
Product Specific Protocols | |
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WB protocol for ATM antibody 83608-5-RR | Download protocol |
IHC protocol for ATM antibody 83608-5-RR | Download protocol |
IF protocol for ATM antibody 83608-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |