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ATP13A1 Polyclonal antibody
ATP13A1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16244-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, rat liver tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 12 publications below |
CoIP | See 1 publications below |
Product Information
16244-1-AP targets ATP13A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ATP13A1 fusion protein Ag9174 相同性解析による交差性が予測される生物種 |
Full Name | ATPase type 13A1 |
Calculated molecular weight | 976 aa, 109 kDa |
Observed molecular weight | 120-130 kDa |
GenBank accession number | BC009302 |
Gene symbol | ATP13A1 |
Gene ID (NCBI) | 57130 |
RRID | AB_2290293 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
IHC protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
IF protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
IP protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.
| ||
Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology | ||
J Cell Sci Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex.
| ||
J Biol Chem The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation. | ||
Cell Rep CATP-8/P5A ATPase Regulates ER Processing of the DMA-1 Receptor for Dendritic Branching. |