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  • Featured Product
  • KD/KO Validated

ATP1A1 Polyclonal antibody

ATP1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14418-1-AP

Synonyms

Na+/K+ ATPase 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1

"ATP1A1 Antibodies" Comparison

View side-by-side comparison of ATP1A1 antibodies from other vendors to find the one that best suits your research needs.
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  • Western Blot (WB) analysis of various lysates using ATP1A1 Polyclonal antibody (14418-1-AP)

    WB analysis using 14418-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATP1A1 Polyclonal antibody (14418-1-AP)

    WB analysis using 14418-1-AP

    37°C incubated HEK-293 membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATP1A1 Polyclonal antibody (14418-1-AP)

    WB analysis using 14418-1-AP

    37°C incubated mouse brain total extract lysate and membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    WB analysis of HEK-293 using 14418-1-AP

    WB result of ATP1A1 antibody (14418-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1 transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    IP experiment of HEK-293 using 14418-1-AP

    IP result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:20000) with HEK-293 cells lysate 1470 ug.

  • Immunoprecipitation (IP) experiment of A431 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    IP experiment of A431 using 14418-1-AP

    IP result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:5000) with A431 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human liver cancer using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human ovary tumor using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human cervical cancer using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human liver cancer using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human colon using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human colon using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human ovary tumor using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human ovary tumor using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human ovary tumor using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    IF Staining of Caco-2 using 14418-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    IF Staining of HEK-293 using 14418-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    FC experiment of HEK-293 using 14418-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1 Polyclonal antibody (14418-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse heart tissue, HEK-293 cells, 37°C incubated HEK-293 cells, 37°C incubated mouse brain tissue, rat heart tissue
Positive IP detected inHEK-293 cells, A431 cells
Positive IHC detected inhuman ovary tumor tissue, human cervical cancer tissue, human colon tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells, HEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14418-1-AP targets ATP1A1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1A1 fusion protein Ag5763 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight 113 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC050359
Gene symbol ATP1A1
Gene ID (NCBI) 476
RRIDAB_2227873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Protocols

Product Specific Protocols
WB protocol for ATP1A1 antibody 14418-1-APDownload protocol
IHC protocol for ATP1A1 antibody 14418-1-APDownload protocol
IF protocol for ATP1A1 antibody 14418-1-APDownload protocol
IP protocol for ATP1A1 antibody 14418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Low-dose metformin targets the lysosomal AMPK pathway through PEN2.

Authors - Teng Ma
View Article
mouseWB

Immunity

Disruption of the Na+/K+-ATPase-purinergic P2X7 receptor complex in microglia promotes stress-induced anxiety

Authors - Songqiang Huang
View Article
WB

Adv Mater

Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction

Authors - Dingyi Wang
View Article
mouseWB

ACS Nano

Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer

Authors - Lingtong Meng
View Article
WB

Adv Sci (Weinh)

Ultrasound Responsive Nanovaccine Armed with Engineered Cancer Cell Membrane and RNA to Prevent Foreseeable Metastasis

Authors - Wenqi Sun
View Article
humanWB

Theranostics

Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer

Authors - Chen Li
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Jimmy (Verified Customer) (02-27-2024)

Strong signal against human cell lysate, but target protein also show up in cell membrane fraction.

  • Applications: Western Blot
FH

Aleix (Verified Customer) (04-26-2022)

We tested it in immunofluorescence with skin frozen sections. It worked, although the signal is weak.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Skin
FH

Gongsheng (Verified Customer) (12-18-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human epithelium
ATP1A1 Antibody Immunofluorescence, validation (1:100 dilution) in Human epithelium (Cat no:14418-1-AP)