Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of HEK-293 using 55187-1-AP
WB result of ATP1A1-Specific antibody (55187-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1-Specific transfected HEK-293 cells.
WB analysis of mouse brain using 55187-1-AP
37°C incubated mouse brain membrane extract lysate and 37°C incubated mouse heart membrane extract lysate were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis using 55187-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 55187-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 55187-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 55187-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55187-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 55187-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 55187-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1-Specific antibody (55187-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of Caco-2 using 55187-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 55187-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1-Specific Polyclonal antibody (55187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ATP1A1-Specific Polyclonal antibody

ATP1A1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 55187-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

ATP1A1, ATP1A 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	37°C incubated mouse brain tissue, HEK-293 cells, HepG2 cells, MCF-7 cells, 37°C incubated mouse heart tissue, Neuro-2a cells
Positive IHC detected in	human ovary cancer tissue, human colon tissue, human kidney tissue, human liver cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells, Caco-2 cells
Positive FC (Intra) detected in	HEK-293 cells

For optimal WB detection with 55187-1-AP, we do not recommend boiling the sample after lysis.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 13 publications below
IF	See 7 publications below

Product Information

55187-1-AP targets ATP1A1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight	113 kDa
Observed molecular weight	100-110 kDa
GenBank accession number	NM_000701
Gene Symbol	ATP1A1
Gene ID (NCBI)	476
ENSEMBL Gene ID	ENSG00000163399
RRID	AB_10859261
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05023
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.

Protocols

Product Specific Protocols
WB protocol for ATP1A1-Specific antibody 55187-1-AP	Download protocol
IHC protocol for ATP1A1-Specific antibody 55187-1-AP	Download protocol
IF protocol for ATP1A1-Specific antibody 55187-1-AP	Download protocol
FC protocol for ATP1A1-Specific antibody 55187-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Autophagy Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury Authors - Yanping Li View Article
mouse	WB	Hypertension Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension. Authors - Shuai Shao View Article
mouse	IF	Mol Biol Cell Retinoschisin is linked to retinal Na/K-ATPase signaling and localization. Authors - Karolina Plössl View Article
human	IF	Sci Rep Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification. Authors - Yan Xu View Article
human	WB	PLoS One A Novel Bufalin Derivative Exhibited Stronger Apoptosis-Inducing Effect than Bufalin in A549 Lung Cancer Cells and Lower Acute Toxicity in Mice. Authors - Miao Liu View Article
human	WB	Exp Eye Res CHIR99021 balance TGFβ1 induced human corneal endothelial-to-mesenchymal transition to favor corneal endothelial cell proliferation. Authors - Yiran Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 55187-1-AP

WB analysis of mouse brain using 55187-1-AP

WB analysis using 55187-1-AP

WB analysis of mouse heart using 55187-1-AP

WB analysis of Neuro-2a using 55187-1-AP

WB analysis of MCF-7 using 55187-1-AP

WB analysis of HepG2 using 55187-1-AP

WB analysis of HEK-293 using 55187-1-AP

IHC staining of human liver cancer using 55187-1-AP

IHC staining of mouse kidney using 55187-1-AP

IHC staining of human liver cancer using 55187-1-AP

IHC staining of human colon using 55187-1-AP

IHC staining of human kidney using 55187-1-AP

IHC staining of human ovary cancer using 55187-1-AP

IHC staining of mouse kidney using 55187-1-AP

IHC staining of mouse kidney using 55187-1-AP

IF Staining of HEK-293 using 55187-1-AP

IF Staining of Caco-2 using 55187-1-AP

FC experiment of HEK-293 using 55187-1-AP

ATP1A1-Specific Polyclonal antibody

ATP1A1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

For optimal WB detection with 55187-1-AP, we do not recommend boiling the sample after lysis.

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury

Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification.

A Novel Bufalin Derivative Exhibited Stronger Apoptosis-Inducing Effect than Bufalin in A549 Lung Cancer Cells and Lower Acute Toxicity in Mice.

CHIR99021 balance TGFβ1 induced human corneal endothelial-to-mesenchymal transition to favor corneal endothelial cell proliferation.