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ATP1A1-Specific Polyclonal antibody

ATP1A1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 55187-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

ATP1A1, ATP1A 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected in37°C incubated mouse brain tissue, HEK-293 cells, HepG2 cells, MCF-7 cells, 37°C incubated mouse heart tissue, Neuro-2a cells
Positive IHC detected inhuman ovary cancer tissue, human colon tissue, human kidney tissue, human liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, Caco-2 cells
Positive FC (Intra) detected inHEK-293 cells

For optimal WB detection with 55187-1-AP, we do not recommend boiling the sample after lysis.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55187-1-AP targets ATP1A1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight 113 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberNM_000701
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
RRIDAB_10859261
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.

Protocols

Product Specific Protocols
WB protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
IHC protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
IF protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
FC protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Autophagy

Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury

Authors - Yanping Li
mouseWB

Hypertension

Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.

Authors - Shuai Shao
mouseIF

Mol Biol Cell

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Authors - Karolina Plössl
humanIF

Sci Rep

Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification.

Authors - Yan Xu
humanWB

PLoS One

A Novel Bufalin Derivative Exhibited Stronger Apoptosis-Inducing Effect than Bufalin in A549 Lung Cancer Cells and Lower Acute Toxicity in Mice.

Authors - Miao Liu
humanWB

Exp Eye Res

CHIR99021 balance TGFβ1 induced human corneal endothelial-to-mesenchymal transition to favor corneal endothelial cell proliferation.

Authors - Yiran Wang
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