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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP1A1 Recombinant antibody, PBS Only

ATP1A1 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240160B5

Cat no : 83191-5-PBS

Synonyms

Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1A1 Recombinant antibody (83191-5-RR)

    IF Staining of A431 using 83191-5-RR (same clone as 83191-5-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1A1 Recombinant antibody (83191-5-RR)

    IF Staining of A431 using 83191-5-RR (same clone as 83191-5-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using ATP1A1 Recombinant antibody (83191-5-RR)

    FC experiment of HEK-293 using 83191-5-RR (same clone as 83191-5-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug ATP1A1 Recombinant antibody (83191-5-RR, Clone:240160B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83191-5-RR

    Affinity and Kinetic Characterization of 83191-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83191-5-RR against Human ATP1A1 were performed. The affinity constant is 87.7 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83191-5-PBS is ATP1A1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP1A1 fusion protein Ag5763 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight 113 kDa
GenBank accession numberBC050359
Gene symbol ATP1A1
Gene ID (NCBI) 476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.