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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1A1 Recombinant antibody (83191-6-RR)

    WB analysis using 83191-6-RR (same clone as 83191-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83191-6-RR (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83191-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP1A1 Recombinant antibody (83191-6-RR)

    IF Staining of HEK-293 using 83191-6-RR (same clone as 83191-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP1A1 antibody (83191-6-RR, Clone: 240160E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1A1 Recombinant antibody (83191-6-RR)

    IF Staining of A431 using 83191-6-RR (same clone as 83191-6-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-6-RR, Clone: 240160E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using ATP1A1 Recombinant antibody (83191-6-RR)

    FC experiment of HEK-293 using 83191-6-RR (same clone as 83191-6-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1 Recombinant antibody (83191-6-RR, Clone:240160E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83191-6-PBS in a different storage buffer formulation.

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ATP1A1 Recombinant antibody, PBS Only

ATP1A1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83191-6-PBS
Clone No.240160E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83191-6-PBS targets ATP1A1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP1A1 fusion protein Ag5763 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight 113 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05023
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.