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  • Featured Product
  • KD/KO Validated

ATP1A2-Specific Polyclonal antibody

ATP1A2-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55179-1-AP

Synonyms

ATP1A2, ATP1A2-Specific, FHM2, KIAA0778, MHP2, Sodium pump subunit alpha 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ATP1A2-Specific Polyclonal antibody (55179-1-AP)

    WB analysis of mouse brain using 55179-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ATP1A2-Specific Polyclonal antibody (55179-1-AP)

    WB analysis of human brain using 55179-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ATP1A2-Specific Polyclonal antibody (55179-1-AP)

    IP experiment of mouse brain using 55179-1-AP

    IP result of anti-ATP1A2-Specific (IP:55179-1-AP, 4ug; Detection:55179-1-AP 1:800) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ATP1A2-Specific Polyclonal antibody (55179-1-AP)

    IHC staining of human skeletal muscle using 55179-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ATP1A2-Specific Polyclonal antibody (55179-1-AP)

    IHC staining of human skeletal muscle using 55179-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ATP1A2-Specific Polyclonal antibody (55179-1-AP)

    IHC staining of mouse heart using 55179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ATP1A2-Specific Polyclonal antibody (55179-1-AP)

    IHC staining of mouse heart using 55179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55179-1-AP targets ATP1A2-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Calculated molecular weight 112 kDa
Observed molecular weight 100 kDa
GenBank accession numberNM_000702
Gene symbol ATP1A2
Gene ID (NCBI) 477
RRIDAB_2881284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1A2, also named as KIAA0778, belongs to the cation transport ATPase (P-type) family and Type IIC subfamily. It is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). Defects in ATP1A2 are a cause of alternating hemiplegia of childhood (AHC). This antibody is specific to ATP1A2.

Protocols

Product Specific Protocols
WB protocol for ATP1A2-Specific antibody 55179-1-APDownload protocol
IHC protocol for ATP1A2-Specific antibody 55179-1-APDownload protocol
IP protocol for ATP1A2-Specific antibody 55179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Calcium

α2 isoform of Na+,K+-ATPase via Na+,Ca2+ exchanger modulates myelin basic protein synthesis in oligodendrocyte lineage cells in vitro.

Authors - Jens Hammann
  • KD Validated
View Article
ratWB

Cell Calcium

Na+/Ca2+ exchanger isoform 1 (NCX1) and canonical transient receptor potential channel 6 (TRPC6) are recruited by STIM1 to mediate Store-Operated Calcium Entry in primary cortical neurons.

Authors - Valentina Tedeschi
View Article