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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP1A3 (middle) Polyclonal antibody

ATP1A3 (middle) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28030-1-AP

Synonyms

ATP1A3, ATP1A3 (middle), DYT12, RDP, Sodium pump subunit alpha 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ATP1A3 (middle) Polyclonal antibody (28030-1-AP)

    WB analysis of HeLa using 28030-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28030-1-AP (ATP1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATP1A3 (middle) Polyclonal antibody (28030-1-AP)

    WB analysis using 28030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28030-1-AP (ATP1A3 (middle) antibody) at dilution of 1:14000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ATP1A3 (middle) Polyclonal antibody (28030-1-AP)

    IHC staining of human prostate cancer using 28030-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28030-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ATP1A3 (middle) Polyclonal antibody (28030-1-AP)

    IHC staining of human prostate cancer using 28030-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28030-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP1A3 (middle) Polyclonal antibody (28030-1-AP)

    IF Staining of HeLa using 28030-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 28030-1-AP (ATP1A3 (middle) antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, SKOV-3 cells, U-87 MG cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28030-1-AP targets ATP1A3 (middle) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1A3 (middle) fusion protein Ag27705 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 3 polypeptide
Calculated molecular weight 113 kDa
Observed molecular weight 113 kDa
GenBank accession numberBC015566
Gene symbol ATP1A3
Gene ID (NCBI) 478
RRIDAB_2881041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na+/K+ homeostasis. This antibody is generated agaist the middle region (378-580aa) of ATP1A3 and detects 113 kDa protein in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)

Protocols

Product Specific Protocols
WB protocol for ATP1A3 (middle) antibody 28030-1-APDownload protocol
IHC protocol for ATP1A3 (middle) antibody 28030-1-APDownload protocol
IF protocol for ATP1A3 (middle) antibody 28030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Sci Adv

De novo ATP1A3 variants cause polymicrogyria.

Authors - Satoko Miyatake
View Article
humanWB

Nat Commun

Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.

Authors - Yi-Ing Chen
View Article