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Validation Data Gallery

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  • Western Blot (WB) analysis of C2C12 cells using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    WB analysis of C2C12 using 10868-1-AP (same clone as 10868-1-PBS)

    C2C12 cell were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    WB analysis using 10868-1-AP (same clone as 10868-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    WB analysis using 10868-1-AP (same clone as 10868-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IHC staining of human prostate cancer using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IHC staining of human prostate cancer using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IHC staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IHC staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IHC staining of mouse skeletal muscle using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IHC staining of mouse skeletal muscle using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IHC staining of human cerebellum using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IHC staining of human cerebellum using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IF Staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ATP1A3 (C-terminal) Polyclonal antibody (10868-1-AP)

    IF Staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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ATP1A3 (C-terminal) Polyclonal antibody, PBS Only

ATP1A3 (C-terminal) Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 10868-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10868-1-PBS targets ATP1A3 (C-terminal) in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1A3 (C-terminal) fusion protein Ag1313 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 3 polypeptide
Calculated molecular weight 113 kDa
Observed molecular weight 100-113 kDa
GenBank accession numberBC015566
Gene Symbol ATP1A3
Gene ID (NCBI) 478
RRIDAB_2060962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na+/K+ homeostasis. This antibody is generated against the C-terminal region (665-1013aa) of ATP1A3 and detects the band around 100-113 kDa in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)