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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1B1 Recombinant antibody (84707-5-RR)

    WB analysis using 84707-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84707-5-RR (ATP1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84707-5-RR

    Affinity and Kinetic Characterization of 84707-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84707-5-RR against Human ATP1B1 were performed. The affinity constant is below 1 pM.

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ATP1B1 Recombinant antibody

ATP1B1 Recombinant Antibody for WB, ELISA
Cat No. 84707-5-RR
Clone No.242109D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase, ATP1B 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, rat heart tissue, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84707-5-RR targets ATP1B1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP1B1 fusion protein Ag7279 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, beta 1 polypeptide
Calculated molecular weight 35 kDa
Observed molecular weight 45-52 kDa
GenBank accession numberBC000006
Gene Symbol ATP1B1
Gene ID (NCBI) 481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05026
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

Protocols

Product Specific Protocols
WB protocol for ATP1B1 antibody 84707-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols