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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1B3 Polyclonal antibody (11142-1-AP)

    WB analysis using 11142-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11142-1-AP (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ATP1B3 Polyclonal antibody (11142-1-AP)

    IHC staining of human prostate cancer using 11142-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11142-1-AP (ATP1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ATP1B3 Polyclonal antibody (11142-1-AP)

    IHC staining of human prostate cancer using 11142-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11142-1-AP (ATP1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP1B3 Polyclonal antibody (11142-1-AP)

    IHC staining of human liver cancer using 11142-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11142-1-AP (ATP1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP1B3 Polyclonal antibody (11142-1-AP)

    IHC staining of human liver cancer using 11142-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11142-1-AP (ATP1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ATP1B3 Polyclonal antibody

ATP1B3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11142-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, CD298, ATPB-3, ATPB 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, THP-1 cells
Positive IHC detected inhuman prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11142-1-AP targets ATP1B3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1B3 fusion protein Ag1589 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, beta 3 polypeptide
Calculated molecular weight 32 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberBC011835
Gene Symbol ATP1B3
Gene ID (NCBI) 483
RRIDAB_2274459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54709
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

Protocols

Product Specific Protocols
WB protocol for ATP1B3 antibody 11142-1-APDownload protocol
IHC protocol for ATP1B3 antibody 11142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis

Authors - Fangquan Chen
View Article