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  • Featured Product
  • KD/KO Validated

ATP1B3 Monoclonal antibody, PBS Only

ATP1B3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9C1

Cat no : 67554-1-PBS

Synonyms

Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, CD298, ATPB-3, ATPB 3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of Jurkat using 67554-1-Ig (same clone as 67554-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of THP-1 using 67554-1-Ig (same clone as 67554-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of human placenta using 67554-1-Ig (same clone as 67554-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of HeLa using 67554-1-Ig (same clone as 67554-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    WB analysis of HepG2 using 67554-1-Ig (same clone as 67554-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IHC staining of human colon using 67554-1-Ig (same clone as 67554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IHC staining of human colon using 67554-1-Ig (same clone as 67554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IHC staining of human lung cancer using 67554-1-Ig (same clone as 67554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IHC staining of human lung cancer using 67554-1-Ig (same clone as 67554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1B3 Monoclonal antibody (67554-1-Ig)

    IF Staining of A431 using 67554-1-Ig (same clone as 67554-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1B3 antibody (67554-1-Ig, Clone: 1H9C1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67554-1-PBS targets ATP1B3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP1B3 fusion protein Ag30127 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, beta 3 polypeptide
Calculated molecular weight 32 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberBC011835
Gene symbol ATP1B3
Gene ID (NCBI) 483
RRIDAB_2882768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).