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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP2A1 Recombinant antibody

ATP2A1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241500D12

Cat no : 84213-5-RR

Synonyms

SERCA1, EC:7.2.2.10, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, ATP2A 1, ATP2A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP2A1 Recombinant antibody (84213-5-RR)

    WB analysis using 84213-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84213-5-RR (ATP2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ATP2A1 Recombinant antibody (84213-5-RR)

    IHC staining of mouse skeletal muscle using 84213-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84213-5-RR (ATP2A1 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84213-5-RR

    Affinity and Kinetic Characterization of 84213-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84213-5-RR against Human ATP2A1 were performed. The affinity constant is 6.90 nM.

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84213-5-RR is ATP2A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP2A1 fusion protein Ag17944 相同性解析による交差性が予測される生物種
Full Name ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
Calculated molecular weight 1001 aa, 110 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC037354
Gene symbol ATP2A1
Gene ID (NCBI) 487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP2A1 also known as SERCA1, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen and is involved in muscular excitation and contraction. Mutations in ATP2A1 cause some autosomal recessive forms of Brody disease(PMID: 23911890, 10914677).

Protocols

Product Specific Protocols
WB protocol for ATP2A1 antibody 84213-5-RRDownload protocol
IHC protocol for ATP2A1 antibody 84213-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols