Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat stomach tissue, mouse stomach tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83079-5-RR targets ATP4A in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, H+/K+ exchanging, alpha polypeptide |
| Calculated molecular weight | 114 kDa |
| Observed molecular weight | 114 kDa |
| GenBank accession number | NM_000704 |
| Gene Symbol | ATP4A |
| Gene ID (NCBI) | 495 |
| RRID | AB_3670796 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20648 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. ATP4A belongs to a family of P-type cation-transporting ATPases, encodes a catalytic alpha subunit of the gastric H+, K+-ATPase, and transports H+ ions in exchange for K+ ions across the apical membrane of parietal cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP4A antibody 83079-5-RR | Download protocol |
| FC protocol for ATP4A antibody 83079-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |