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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP4B Polyclonal antibody

ATP4B Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 18692-1-AP

Synonyms

Potassium-transporting ATPase subunit beta, H+/K+ ATPase beta, ATP4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP4B Polyclonal antibody (18692-1-AP)

    WB analysis using 18692-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using ATP4B Polyclonal antibody (18692-1-AP)

    WB analysis of mouse colon using 18692-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using ATP4B Polyclonal antibody (18692-1-AP)

    WB analysis of rat kidney using 18692-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ATP4B Polyclonal antibody (18692-1-AP)

    IHC staining of human stomach cancer using 18692-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18692-1-AP (ATP4B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse stomach tissue using ATP4B Polyclonal antibody (18692-1-AP)

    IF Staining of mouse stomach using 18692-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using ATP4B antibody (18692-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse stomach tissue, mouse colon tissue, rat kidney tissue, rat stomach
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

18692-1-AP targets ATP4B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP4B fusion protein Ag6935 相同性解析による交差性が予測される生物種
Full Name ATPase, H+/K+ exchanging, beta polypeptide
Calculated molecular weight 33 kDa
Observed molecular weight 60-80 kDa
GenBank accession numberBC029059
Gene symbol ATP4B
Gene ID (NCBI) 496
RRIDAB_10597241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATP4B antibody 18692-1-APDownload protocol
IHC protocol for ATP4B antibody 18692-1-APDownload protocol
IF protocol for ATP4B antibody 18692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

FASEB J

Palmatine ameliorates N-methyl-N'-nitrosoguanidine-induced chronic atrophic gastritis through the STAT1/CXCL10 axis

Authors - Yuan Zhou
View Article