Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, NIH/3T3 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human lung cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IF | See 1 publications below |
Product Information
14893-1-AP targets ATP5D in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATP5D fusion protein Ag6682 相同性解析による交差性が予測される生物種 |
| Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit |
| Calculated molecular weight | 17 kDa |
| Observed molecular weight | 15-17 kDa |
| GenBank accession number | BC002389 |
| Gene Symbol | ATP5D |
| Gene ID (NCBI) | 513 |
| RRID | AB_2274510 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30049 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP5D antibody 14893-1-AP | Download protocol |
| IHC protocol for ATP5D antibody 14893-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages. | ||
Int J Mol Sci CA9 Silencing Promotes Mitochondrial Biogenesis, Increases Putrescine Toxicity and Decreases Cell Motility to Suppress ccRCC Progression. | ||
Front Aging Neurosci Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders. | ||
Am J Physiol Cell Physiol 3-MST/H2S protects cerebral endothelial cells against OGD/R-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway. | ||
J Neuropathol Exp Neurol Altered Mitochondria, Protein Synthesis Machinery, and Purine Metabolism Are Molecular Contributors to the Pathogenesis of Creutzfeldt-Jakob Disease. | ||
Cell Death Dis Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability. |