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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP5D Monoclonal antibody, PBS Only

ATP5D Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12C7

Cat no : 66673-1-PBS

Synonyms

ATP5D, F ATPase delta subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP5D Monoclonal antibody (66673-1-Ig)

    WB analysis using 66673-1-Ig (same clone as 66673-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66673-1-Ig (ATP5D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66673-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP5D Monoclonal antibody (66673-1-Ig)

    IHC staining of human kidney using 66673-1-Ig (same clone as 66673-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66673-1-Ig (ATP5D antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66673-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP5D Monoclonal antibody (66673-1-Ig)

    IHC staining of human kidney using 66673-1-Ig (same clone as 66673-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66673-1-Ig (ATP5D antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66673-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66673-1-PBS targets ATP5D in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5D fusion protein Ag7016 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
Calculated molecular weight 17 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC002389
Gene symbol ATP5D
Gene ID (NCBI) 513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.