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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP5F1 Monoclonal antibody (68304-1-Ig)

    WB analysis using 68304-1-Ig (same clone as 68304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68304-1-Ig (ATP5F1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68304-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP5F1 Monoclonal antibody (68304-1-Ig)

    IF Staining of HeLa using 68304-1-Ig (same clone as 68304-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (68304-1-Ig, Clone: 1A10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68304-1-PBS in a different storage buffer formulation.

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ATP5F1 Monoclonal antibody, PBS Only

ATP5F1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68304-1-PBS
Clone No.1A10G9

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ATP5F1, ATPase subunit b, PIG47

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68304-1-PBS targets ATP5F1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5F1 fusion protein Ag8571 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
Calculated molecular weight 256 aa, 29 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC005366
Gene Symbol ATP5F1
Gene ID (NCBI) 515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP24539
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.