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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATP5G3 Polyclonal antibody (21662-1-AP)

    IHC staining of human ovary tumor using 21662-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21662-1-AP (ATP5G3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATP5G3 Polyclonal antibody (21662-1-AP)

    IHC staining of human ovary tumor using 21662-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21662-1-AP (ATP5G3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ATP5G3 Polyclonal antibody

ATP5G3 Polyclonal Antibody for IHC, ELISA
Cat No. 21662-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

ATP synthase proteolipid P3, ATP5G3, ATPase protein 9, ATPase subunit c, P3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21662-1-AP targets ATP5G3 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5G3 fusion protein Ag16406 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9)
Calculated molecular weight 142 aa, 15 kDa
GenBank accession numberBC106881
Gene Symbol ATP5G3
Gene ID (NCBI) 518
RRIDAB_3085665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48201
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5G3, also known as ATP5MC3, belongs to the ATPase C chain family. ATP5G3 encodes subunit 9 (ATPase subunit c), which is a subunit of the multi-subunit enzyme that catalyzes ATP synthesis during oxidative phosphorylation in mitochondria. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The subunit c is a component of the proton channel proteins in the F0 portion of the enzyme.

Protocols

Product Specific Protocols
IHC protocol for ATP5G3 antibody 21662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols