Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15842-1-AP targets ATP5J2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATP5J2 fusion protein Ag8598 相同性解析による交差性が予測される生物種 |
| Full Name | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2 |
| Calculated molecular weight | 5-11 kDa |
| Observed molecular weight | 11kDa |
| GenBank accession number | BC003678 |
| Gene Symbol | ATP5J2 |
| Gene ID (NCBI) | 9551 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56134 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP5J2 (also known as ATP synthase-coupling factor 6, or CF6) is a nuclear-encoded subunit of mitochondrial ATP synthase (Complex V), the enzyme responsible for synthesizing ATP from ADP and inorganic phosphate (Pi) during oxidative phosphorylation (OXPHOS).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP5J2 antibody 15842-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |