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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP5J2 Monoclonal antibody, PBS Only

ATP5J2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B3C3

Cat no : 68128-1-PBS

Synonyms

ATP5J2, ATP5JL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP5J2 Monoclonal antibody (68128-1-Ig)

    WB analysis using 68128-1-Ig (same clone as 68128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATP5J2 Monoclonal antibody (68128-1-Ig)

    WB analysis using 68128-1-Ig (same clone as 68128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit heart tissue using ATP5J2 Monoclonal antibody (68128-1-Ig)

    WB analysis of rabbit heart using 68128-1-Ig (same clone as 68128-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATP5J2 Monoclonal antibody (68128-1-Ig)

    WB analysis using 68128-1-Ig (same clone as 68128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ATP5J2 Monoclonal antibody (68128-1-Ig)

    IF Staining of MCF-7 using 68128-1-Ig (same clone as 68128-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATP5J2 antibody (68128-1-Ig, Clone: 2B3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68128-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68128-1-PBS targets ATP5J2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5J2 fusion protein Ag8811 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
Calculated molecular weight 5-11 kDa
Observed molecular weight12 kDa
GenBank accession numberBC003678
Gene symbol ATP5J2
Gene ID (NCBI) 9551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.