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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP5L Monoclonal antibody

ATP5L Monoclonal Antibody for IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D10F9

Cat no : 60434-1-Ig

Synonyms

ATPase subunit g, ATP5MG, ATP5JG, ATP synthase membrane subunit g, 2D10F9

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATP5L Monoclonal antibody (60434-1-Ig)

    IF Staining of HepG2 using 60434-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5L antibody (60434-1-Ig, Clone: 2D10F9 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60434-1-Ig targets ATP5L in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5L fusion protein Ag9287 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G
Calculated molecular weight 11 kDa
GenBank accession numberBC015128
Gene symbol ATP5L
Gene ID (NCBI) 10632
RRIDAB_3670259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G Magarose purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5L gene encodes ATP synthase subunit g of the Fo complex.

Protocols

Product Specific Protocols
IF protocol for ATP5L antibody 60434-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols