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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP5O Monoclonal antibody, PBS Only

ATP5O Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D3B8

Cat no : 66696-1-PBS

Synonyms

ATP5O, ATPO, OSCP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP5O Monoclonal antibody (66696-1-Ig)

    WB analysis using 66696-1-Ig (same clone as 66696-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66696-1-Ig (ATP5O antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP5O Monoclonal antibody (66696-1-Ig)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP5O Monoclonal antibody (66696-1-Ig)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP5O Monoclonal antibody (66696-1-Ig)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ATP5O Monoclonal antibody (66696-1-Ig)

    IHC staining of human colon cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ATP5O Monoclonal antibody (66696-1-Ig)

    IHC staining of human colon cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP5O Monoclonal antibody (66696-1-Ig)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP5O Monoclonal antibody (66696-1-Ig)

    IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66696-1-Ig (ATP5O antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP5O Monoclonal antibody (66696-1-Ig)

    IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66696-1-Ig (ATP5O antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66696-1-PBS targets ATP5O in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5O fusion protein Ag1458 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Calculated molecular weight 23 kDa
Observed molecular weight 23-25 kDa
GenBank accession numberBC021233
Gene symbol ATP5O
Gene ID (NCBI) 539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.