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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP6 Polyclonal antibody, PBS Only

ATP6 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55313-1-PBS

Synonyms

MT-ATP6, MTATP6, MT ATP6, ATPASE6, ATP 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6 Polyclonal antibody (55313-1-AP)

    WB analysis using 55313-1-AP (same clone as 55313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55313-1-AP (ATP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C2C12 cells using ATP6 Polyclonal antibody (55313-1-AP)

    WB analysis of C2C12 using 55313-1-AP (same clone as 55313-1-PBS)

    C2C12 cell were subjected to SDS PAGE followed by western blot with 55313-1-AP (ATP6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55313-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55313-1-PBS targets ATP6 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ATP synthase 6; ATPase subunit 6
Calculated molecular weight 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberJ01415
Gene symbol ATP6
Gene ID (NCBI) 4508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATP synthase, also known as FoF1 complex, is a critical mitochondrial OXPHOS enzyme involved in the regulation of mitochondrial ATP production and in the maintenance of the mitochondrial membrane potential. It is composed of three components (F1, Fo and the peripheral stalk). F1, the soluble catalytic core, is above the membrane, inside the matrix of the mitochondria; consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon); Fo, comprising the proton channel, is within the membrane; Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP6 is the a subunit of Fo region.