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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP6 Recombinant antibody, PBS Only

ATP6 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240041B6

Cat no : 83201-3-PBS

Synonyms

MT-ATP6, MTATP6, MT ATP6, ATPASE6, ATP 6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP6 Recombinant antibody (83201-3-RR)

    IF Staining of HeLa using 83201-3-RR (same clone as 83201-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP6 antibody (83201-3-RR, Clone: 240041B6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83201-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using ATP6 Recombinant antibody (83201-3-RR)

    FC experiment of U2OS using 83201-3-RR (same clone as 83201-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug ATP6 Recombinant antibody (83201-3-RR, Clone:240041B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83201-3-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83201-3-PBS targets ATP6 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ATP synthase 6; ATPase subunit 6
Calculated molecular weight 25 kDa
GenBank accession numberJ01415
Gene symbol ATP6
Gene ID (NCBI) 4508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATP synthase, also known as FoF1 complex, is a critical mitochondrial OXPHOS enzyme involved in the regulation of mitochondrial ATP production and in the maintenance of the mitochondrial membrane potential. It is composed of three components (F1, Fo and the peripheral stalk). F1, the soluble catalytic core, is above the membrane, inside the matrix of the mitochondria; consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon); Fo, comprising the proton channel, is within the membrane; Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP6 is the a subunit of Fo region.